hIPSC - Disease Modeling

Introduction

iPSCs derived by the reprogramming of somatic cells from diseased individuals,represent an important new platform for the development of human disease models. Upon appropriate differentiation these cells can then be used to study normal and pathologic human tissue development in vitro, enabling new insights into disease pathology as well as the development of novel therapeutic agents and patient-specific cell replacement therapies.

Knowledge Transfer

A key activity of the core facility is to offer advice and direction for in vitro disease modeling and to provide a network of collaborators with complementary expertise in differentiation protocols and phenotyping analyses.

List of affiliated institutions and collaborators:

Human Induced Pluripotent Stem Cells Initiative

Wellcome Trust Sanger Institute

Cambridge Stem Cells Institute

iPSC Derivation and Validation Services

The core facility has successfully generated high-quality iPSCs from more than 100 patients. Upon request the iPSC core facility can derive iPSCs from dermal fibroblast cultures or peripheral blood mononuclear cells (PBMCs), provided by the investigator, using non-integrating reprogramming techniques. The full package of derivation and validation usually takes up to 3 months and includes:

  •     Expansion of starting population of cells
  •     Mycoplasma testing
  •     Freezing vials and plating for transduction
  •     Transduction of cells
  •     Splitting onto 100mm dishes with irradiated MEFs
  •     Feeding every day
  •     Picking colonies
  •     Expansion of colonies for 10-12 passages (3 lines per patient)
  •     Freezing (3-6 vials per line)

Application for iPSCs derivation services in the category - in vitro disease modeling, download here

iPSCs Derivation Submission Form - available here

Human induced Pluripotent Stem Cells Protocols - pdf download

Training for iPSCs derivation and iPSCs culture

The iPSC Core Facility offers training, which provides hands-on experience with fibroblast reprogramming for the generation of iPSCs, iPSCs maintenance, differentiation, and cryopreservation. Additionally, the facility provides protocols foriPSCs maintenance and differentiation.

The training course is open to both BRC investigators and external investigators. Please contact us for a price list if you are from non-academic institution. Cost includes cells, media, all reagents and training manuals.

We can accommodate up to a six people per training for each training session, which takes four days. Please contact us for available dates and times for training.

R&D projects

Genome Editing Services mediated by homologous recombination facilitated with Transcription Activator-Like Effector Nucleases (TALENs).

Various experimental approaches in disease modeling require precise genome editing, which remains a difficult and inefficient process in iPSCs. TALENs have emerged as a powerful tool to facilitate gene targeting. The modular architecture of TAL effectors theoretically enables targeting of any genomic locus. Thus, this system can be used to generate knock-outiPSCs lines as well as isogenic cell lines for disease modeling.

Contact

For more information or assistance contact Dr Ludovic Vallier: lv225@cam.ac.uk